ZEISS Seminars at Neuroscience 2017
Biological Applications of X-Ray Microscopy and Correlative XRM - FIB-SEM Imaging

X-Ray Microscopy (XRM) is a relatively new technique that combines the geometric magnification of traditional micro-CT with the optical magnification of light microscopy. The ZEISS Versa XRM system allows an unprecedented view inside samples varying in size from the mesoscale (cm) to the microscale (um) at consistently sub-micron image resolutions. This webinar will focus on the biological applications of X-Ray microscopy, covering the imaging of calcified structures (such as bone) to soft tissues such as the intervertebral disc to visualizing blood vessel using vascular tracing agents. The basics of sample preparation will be covered as well as the pros and cons of different imaging conditions. Finally, a sneak view will be provided into using XRM to spatially target, in three-dimensions, tissue specific structures in a whole organism for 3D ultrastructural imaging using Focused Ion Beam - Scanning Electron Microscopy (FIB-SEM) using the ATLAS 5 Correlative Workspace.